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Image Search Results
Journal: PLoS ONE
Article Title: Sensitive Tumorigenic Potential Evaluation of Adult Human Multipotent Neural Cells Immortalized by hTERT Gene Transduction
doi: 10.1371/journal.pone.0158639
Figure Lengend Snippet: The hTERT gene was transduced into 682TL and 779TL ahMNCs using a lentiviral vector. (A) Morphology of the ahMNCs was compared with that of hTERT-ahMNCs. (B) ahMNCs and hTERT-ahMNCs were cultured in vitro for a long time (>18 in vitro passages), and the numbers of cells were traced. (C) hTERT expression was confirmed by RT-PCR. (D) Telomere length was analyzed by qPCR and compared. (E) Expression of the neural stem cell marker Nestin and ahMNCs differentiation potential in vitro was compared with those of hTERT-ahMNCs. ahMNCs or hTERT-ahMNCs were cultured under differentiating conditions for 12 days. MAP2 for neurons and GFAP for astrocytes. BD; Before differentiation, AD; After differentiation.
Article Snippet: The hTERT-overexpressing lentiviral vector was co-transfected with the VSVG and
Techniques: Plasmid Preparation, Cell Culture, In Vitro, Expressing, Reverse Transcription Polymerase Chain Reaction, Marker
Journal: iScience
Article Title: LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells
doi: 10.1016/j.isci.2023.106254
Figure Lengend Snippet:
Article Snippet:
Techniques: Plasmid Preparation, Recombinant, Modification, Protease Inhibitor, SYBR Green Assay, Reporter Assay, Mutagenesis, Microarray, shRNA, Library Screening, Software